WebMar 17, 2016 · Induction of ROS-dependent apoptosis by cisplatin combined with gallic acid (GA) in H446 cells. (A) Apoptosis assay by flow cytometry using PI and Annexin V-FITC double staining. H446 cells were treated with or without 6 mM NAC for 2 h before challenge with 5 µg/ml cisplatin combined with 3 µg/ml GA for 24 h. WebSep 1, 2024 · CDDP had differential effect on proteins expression and induced apoptosis in GC cells. a Western blot shows the effect of CDDP on protein expression in AGS and NCI - N87 cell lines. The expression of tumor suppressor protein 53 increased with CDDP concentration, whilst Akt and NFkB expressions reduced, notice the cleaved PARP …

Cisplatin nephrotoxicity: new insights and therapeutic …

WebMay 16, 2011 · After these metabolic changes a drastic decrease in cellular impedance occurred at about 10–11 h after exposure to cisplatin ( Fig. 2 C ), which reflects the … WebMar 1, 2006 · The chloride ions in cisplatin are particularly important in its action as a drug. They are relatively stable when the drug is outside the cell, where the chloride concentration is normally high. But when the drug gets inside the cell and the chloride concentration drops, cisplatin loses its two chlorides, and they are replaced by water … try try try not to laugh

Effect of naringenin and its combination with cisplatin in cell …

WebApr 28, 2011 · For all cell lines, it is true that concentrations of cisplatin up to 30μM caused mainly a mode of cell death with clear morphological and biochemical characteristics of apoptosis, whereas the commonest phenotype observed with concentrations equal or higher than 300μM had clearly lost many of these characteristics and more closely … WebCisplatin is the most important and efficacious chemotherapeutic agent for the treatment of advanced gastric cancer. Cisplatin forms inter- and intrastrand crosslinked DNA adducts … WebFor apoptosis detection, flow cytometry was used to verify whether TAS induced apoptosis in NSCLC cells. The apoptosis rate was significantly higher in response to an increasing dose of TAS in A549 and H1299 cells (Fig. 2 D-2E). Taken together, these findings suggested that TAS has good anticancer activity. try try try pink