Thermo mirna mimic

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August undefined, 2024

Webb29 sep. 2024 · microRNAs are small non-coding RNAs that regulate several genes post-transcriptionally by complementarity pairing. Since discovery, they have been reported to … WebbmiRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA …

MicroRNA-562 negatively regulated c-MET/AKT pathway in the …

Webb2 dec. 2015 · Next we investigated the turnover time of transfected miRNA mimics by following the Dy547 signal in transfected cells for up to 72 h. At the 100 nM transfection … WebbAssay Name: Stem-loop Accession Number: MI0000893: miRBase Version: v22.1: Mature miRNA Sequence: UAAGGCACGCGGUGAAUGCC: Species: Mouse, Rat, Astatotilapia burtoni ... direct flights from kuwait to kochi

MicroRNA-486-3p directly targets BIK and regulates apoptosis and …

Webb如何选择miRNA对照：锐博生物所有预制mimic NC均与人、小鼠、大鼠基因组无同源性（其它物种可联系我们分析确认与目标实验物种无同源性的NC产品），刚开始相关实验时，建议在预实验中比较不同的NC（如#22、#24、#19等）对检测指标的影响并选择没有影响或影响较小的NC进行正式实验。 WebbThe final concentration of miRNA mimics was 50 nmol/L, the final concentration of miRNA inhibitors was 100 nmol/L, and the final concentration of siRNAs was 20 nmol/L. To generate pGL3-lncRNA 1308 constructs, the sequence of lncRNA 1308 mRNA was amplified by the primers listed in Table S1. WebbSimilarly, the cells were cotransfected with lenti-LINC00888 with or without miR-126 mimics (20 nM). Lipofectamine 3000 (Thermo Fisher Scientific) was used as a … direct flights from lafayette la

mirVana™ miRNA Mimic, miR-1 Positive Control

Mir 206 Mirvana Mirna Mimic Thermo Fisher Bioz

WebbmiRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA … WebbAssay IDMC41146. miRBase ID hsa-miR-12120. miRBase Accession # MIMAT0049014. Species Human. Product Type mir Vana® miRNA mimic. Availability. Made to Order. … forum works llcWebbThis Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals. RUO – Research Use Only direct flights from kuwait to abu dhabi

"Webb21 dec. 2024 · miScript miRNA Mimics are chemically synthesized, double-stranded RNAs which mimic mature endogenous miRNAs after transfection into cells. miScript miRNA … " - Thermo mirna mimic

Thermo mirna mimic

WebbThe miR-562 mimics, inhibitor, and negative control (NC) were purchased from GenePharma (Shanghai, P.R. China). Cells were grown on 80% confluence prior to transfection. Transfection of the miR-562 mimics or inhibitor into glioma U251 and U87 cells was performed by using Lipofectamine 2000 (Invitrogen) according to the … WebbThe miR-126 mimics and inhibitor were provided by GenePharma (Shanghai, China). Tumor xenograft in vivo model Next, 5- to 6-week-old BALB/c nude mice were purchased from the animal center at Vital River (Beijing, China) and maintained under pathogen-free conditions according to the protocols.

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WebbFor transfection, Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) was used. miR486-3p mimics, inhibitors, and negative control were purchased from GenePharma (Shanghai, China). BIK siRNA was synthesized and chemically modified by Santa Cruz Biotechnology (Dallas, TX, USA). Webb30 juni 2024 · The cell suspensions were centrifuged at 500g for 5 min and resuspended in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific) with 10% fetal …

WebbLipofectamine RNAiMAX Transfection Reagent provides the highest transfection efficiencies set the widest variety of cell species available siRNA-mediated gene knockdown experiments. Lipofectamine RNAiMAX … Webb1 jan. 2010 · Schematic presentation of actions of miRNA mimic (miR-Mimic) compared with the miRNA and small interference RNA (siRNA). Synthetic miR-Mimic and siRNA are …

WebbTaqMan Search. Primary Antibodies. Order Status. Quick Order. Sign in. Gene Expression SNP Genotyping Copy Number siRNA MicroRNA Mutation Detection Primer Designer. Webb17 jan. 2024 · Of the seven novel miRNAs, miR-SX4 mimic significantly induced cell cycle arrest at G2/M phase in HEK293 cells (Fig. 1a,b) and HeLa cells (Fig. 1c; Supplementary …

Webb14 apr. 2024 · Among the non-coding RNAs (ncRNAs) that lack protein-coding potential, miRNAs are a class of small endogenous ncRNAs (18–24 nt) that regulate gene expressions by the translational repression or degradation of their targets. There is growing evidence that miRNA-mediated mechanisms also play critical roles in bone development …

WebbHere, we show that a self-assembled dual-colour RNA-triple-helix structure comprising two miRNAs—a miR mimic (tumour suppressor miRNA) and an antagomiR ... affecting … forum workplace inclusionWebbThe following file types are supported: ANSI files with extension .txt, .txtN (where N is a number) or .vcf containing either a list of keywords, the exported results from a previous assay search, or VCF-compliant data. forum world arsonWebbOn the other hand, the interaction of miR‐2834 and lncR26319 was investigated with 4 groups of cells transfected with (1) pmirGlO‐lncR26319, (2) NC and pmirGlO‐lncR26319, (3) miR‐2834 mimics and pmirGlO‐lncR26319, and (4) miR‐2834 mutant mimics (the first 11 nucleotides of miR‐2834 were designed as the complementary sequence and … forum workoutWebbLipofectamine RNAiMAX Transfection Reagent provides the highest transfection efficiencies on this widest breed of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine RNAiMAX is a proprietary RNAi-specific cationic lipid forum workplaceWebb27 apr. 2024 · Cells were transfected with 100 nM of each miRNA mimic (Dharmacon, miRIDIAN microRNA mimics, Thermo Fisher Scientific) according to the manufacturer’s … direct flights from lagosWebb14 aug. 2016 · I am trying to inhibit one miRNA in MDA-MB-231 breast cancer cells using mirVana inhibitors (Thermo Fisher) and lipofectamine. I see efficient transfection since I … forum works milford ohioWebbThermo Fisher mirvana mirna mimic negative control Mirvana Mirna Mimic Negative Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, … forum wotblitz eu