WebFeb 22, 2024 · Names like BenRiach, The Balvenie, The Glenlivet, Lagavulin, Bowmore, and Ardbeg are well-known and beloved for good reason: They’re reliably excellent, no matter … WebDNA amplification was conducted in 25-μl reactions using 12.5 μl of PCR mix (TsingKe Co. Ltd Beijing, China), 1.0 μl of 10 μM each primer, 1.5 μl of template DNA, and 9 μl of ddH 2 O. The amplification program consisted of one cycle at 94 °C for 5 min, followed by 35 cycles at 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min, and one cycle at 72 °C for 5 min (Beebe …
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WebPositive bands were cut out and purified, and then sequenced at TSINGKE Company (Kunming, China), bi-directionally. The sequencing results were checked and assembled using the ContigExpress (independent module from Vector NTI Advance V 11, Invitrogen, USA). Sequence comparison was performed using BLAST analysis ... WebCloning Vector (TransGen Biotech Beijing Co., LTD). Then, the recombinant plasmids transformed into the resistant competent cell. The positive clones were screened and cultured in LB (10 mL) with ampicillin for 5 h. The recombinant plasmids were obtained by plasmid DNA extraction kit of TSINGKE Biological Technology Beijing Co., LTD. iphone repair shop in juhu mumbai
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WebLegal Name Beijing Tsingke Biotechnology Co., Ltd. Company Type For Profit. Contact Email [email protected]. Phone Number 400-668-3730. Tsingke Biotechnology … WebMar 1, 2024 · Ma et al. demonstrate that transcription factor and coactivator p300 can cocondense. They systematically dissect the mechanism and function of co-condensation. The findings offer quantitative insights into how p300 catalytic activity, gene transcriptional bursting, and gene dose-response curve can be regulated by co-condensation. WebAll the lncRNA expressing plasmids were generated by Tsingke company (Beijing, China) unless otherwise specifically mentioned. Shortly, the full length of lncRNA was synthesized by Tsingke company then cloned into the empty vector pcDNA3.1(+) plasmids. The plasmids pcDNA3.1(+)-MAMDC2-AS1 were purchased from Vigene Biosciences (Jinan, … iphone repair shop in karimnagar